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Antimicrobial therapy with broad-spectrum agents is often associated with adverse reactions, such as drug-induced liver injury (DILI), which may be related to induction of liver injury through the activation of immune cells. Endotoxin, a potent macrophage stimulant, plays a pivotal role in the pathogenesis of DILI. Tigecycline is a novel glycylcycline antimicrobial agent with a broad spectrum of antimicrobial activity. In the present study, we investigated the effect of tigecycline on nitric oxide (NO) production in mouse peritoneal macrophages by measuring the levels of NO released from peritoneal macrophages by the Griess reagent. Tigecycline and imipenem significantly inhibited NO production in peritoneal macrophages. In addition, tigecycline inhibited the lipopolysaccharide-induced increase in tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 expression in peritoneal macrophages, whereas imipenem did not. Conversely, tigecycline significantly enhanced the expression of heme oxygenase (HO)-1 in lipopolysaccharide-activated peritoneal macrophages, whereas imipenem did not. Tigecycline decreased the production of reactive oxygen species (ROS) in lipopolysaccharide-stimulated peritoneal macrophages, whereas im 0b46394aab
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